In article <4tlpbs$2hc4 at news.doit.wisc.edu>, klenchin at macc.wisc.edu (Dima
Klenchin) wrote:
> Just curious - what type of anion exchanger is it?
> QAE? And what is the support made of? Simply can't believe
> this is smth magic that qiagen developed. Must be some oldie
> for which a new application was found.
>
My understanding is that it is has charged groups (ie. DEAE?) attached to
a silica matrix. These groups are attached in high density which allows a
greater salt gradient to be used to elute the DNA. Thus you will have less
overlap with proteins and RNA.
I do not find a great advantage of their preps($$) except if I want highly
pure DNA. Time wise is it not much of a savings either. One day when I
have time I want to do the basic miniprep and apply the Na Acetate/DNA
solution to a DEAE sepharose column to see what sticks. I suspect there
will not be much of a difference.
Does anyone else have any ideas?
Glenn
