In article <pharmscience.11.2FCA6EFA at gandalf.otago.ac.nz> pharmscience at gandalf.otago.ac.nz writes:
>In our lab we have had a continiung problem with peptides adsorbing to glass-
>ware. Suggestions to overcome have included washing the containers with a
>surfactant, adding a competing protein (eg 2% BSA), or working at
>(higher) concentrations where losses that do occur are negligible.
We had a similar problem with a particularly hydrophobic protein which stuck
not to glassware but to plasticware (including ultrafiltration units and
PD-10 gel filtration columns, which made it a curse to purify). We solved the
problem by pretreating everything overnight with 5% Tween-20, then washing
thoroughly with water. That's probably not applicable to glassware, but in
view of one other reply to this posting ("use plastic glassware?") maybe not
irrelevant.
Good luck with a sticky problem
Michael Kertesz
ETH-Microbiology
Zurich, Switzerland
