Does anyone have any trick for getting rid of lipids from a total protein
extract? I'm purifying proteins from plant extracts and the lipids really gunk
up the ion-exchange clomns that i use in the first two steps of the
purification. So far my only recourse has been to wash the columns alot with
NaOH, and to pour new ones often, but this is rather expensive (and a pain).
I'm looking for any method that would work for either a small scale (0.5 ml
sample) or large scale (1l)protein prep.
Daniel Chamovitz
chamo at peaplant.biology.yale.edu
