On 18 May 1995, Dr. Jeff Lightfoot wrote:
> What methods are recommended for removing 1% SDS from protein? Is
> dialyzing sufficient? The protein is to be used for crystallography and/or
> nmr. Pierce sells a gel that is supposed to remove detergents, but they
> keep bumping the availability date back. Thanks for your help.
>> Jeff.
Removal of SDS from protein can be accomplished in a number of different
ways:
1. Electrodialysis using a dialysis bag. Ofcourse the surrounding
buffer has to be free of diaysis.
2. Equilibrium dialysis, which you have mentioned.
3. Electroelution into SDS free buffer using Triton X100, where
TritonX100 is removed by dialysis later. (Holloway,('73) Anal. Biochem.,
53:304)
4. Ion retardation columns (Kapp & Vinogradov (78), Anal.
Biochem. 91: 230.
5. Anion Exchange, Weber & Cutter JBC 246:4504.
Hope this helps,
HSR
>>>>>>>>>>>>>>>>>>>>>>>>>>>
Hiranya S. Roychowdhury
Plant Genetic Engineering Lab.
Box 3GL, NM State Univ.
Las Cruces, NM 88003
Phone: (505) 646-5785
hroychow at nmsu.edu
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