Hello
>> sometimes I have to deal with proteins electroblotted onto pvdf
> membrane or in sds page that are impossible to sequence because they
> are blocked at the N terminal end .
> I usually try CNBr cleavage but after that I have problems in the
> extraction of obtained peptides.
The protein sequencing lab here routinely sequences from electroblotted
proteins.
You can look up: Fernandez, J., et al. (1992) Analytical Biochemistry, 201,
255-264.
There is also a more recent reference but it was buried under a desk avalanche
a few days ago, and I cannot find it right now (also by Joe Fernandez, et al.).
Good luck,
Jim
James J. Cheetham
Laboratory of Molecular
and Cellular Neuroscience
The Rockefeller University
cheethj at rockvax.rockefeller.edu
