IUBio

Lysing E,coli in guanidine HCl

Steve Dunn dunnsm at bbsrc.ac.uk
Tue May 16 11:51:12 EST 1995


In article <3pa44m$11d at is.bbsrc.ac.uk>, Andy Phillips <Andy.Phillips at bbsrc.ac.uk> says:
>



Have you tried rapidly freeze-thawing the pellets (dry-ice/EtOH bath)
a couple of times before adding denaturant? I suspect this may resolve
the problem.

Regards, SMD (dunnsm at bbsrc.ac.uk)



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