Hello, netter,
I wish to detect changes in the level of a specific protein expressed in
brain tissue. I have an antibody that will recognize the protein. What is
the best assay I can run to quantitate the change in expression? I don't need
absolute numbers, but I need an assay that will accurately detect changes in
protein levels. I have tried western blots, but I feel this assay has a lot
of room for variation. I would appreciate any suggestions.
-fm