In <D8FqoA.A8s at cc.umontreal.ca> parents at ERE.UMontreal.CA (Parent Stephane) writes:
>Preparation of crude membrane fraction is used routinely in many
>laboratories studying membrane proteins. The centrifugal force used to
>sediment this crude particulate fraction which include the plasma
>membranes vary from an investigator to the next. A rapid survey indicates
>that it varies from 10,000g to 60,000g with 45,000g being used very
>often. Is there any rationale for these differences? Could anybody
>recommand a refference which would be useful to determine the appropriate
>centrifugal force to use ?
I believe the original reference on this subject is Kaback's article
on bacterial membranes. He uses 45,000g. The reference is:
Bacterial Membranes (1971). by H.R. Kaback. in
Methods in Enzymology Vol 22. pp. 99-120.
Actually, I'm not sure that Vol. 22 is correct but the year is 1971.
there are a number of other Methods in Enzymology volumes that deal
with membranes. It should be too much work to skim through a few of
these and get an idea for an average g force used in the majority of
these articles.
--
Alan Kaiser alkaiser at eden.rutgers.edu
Graduate Program in Microbiology and Molecular Genetics
Rutgers University New Brunswick,NJ
