DAVID MISZTAL (P1034294 at CSDVAX.CSD.UNSW.EDU.AU) wrote:
> Someone has asked me to run a protein sample which is in
> normal reducing buffer used for 1D gels. Has anyone ever tried to
> add 2D sample buffer to such a sample to obtain successful separation
> on a 2d gel? Alternatively, is there a method for precipitating
> proteins from reducing buffer?
The problem is probably not the reducing agent but the SDS which will
make isoelectric focusing next to impossible.
--Cornelius.
--
/* Cornelius Krasel, Institut f. Pharmakologie, Versbacher Str. 9, D-97078 */
/* Wuerzburg, Germany email: phak004 at rzbox.uni-wuerzburg.de */
/* "Science is the game you play with God to find out what His rules are." */
