Hi there,
here is the cocktail I use for protein preparations from yeast:
protease inhibitors
100 mM PMSF
1 mM EDTA
1,5 microg/ml Pepstatin
1 mM Benzamidin
2 microg/ml Leupeptin
phosphatase inhibitors
1,25 microM Ocadaic Acid
5 mM Natriumfluoride
others
10 microl/ml NP-40
other protocolls see
Benyon R.J. (1989): "Initial planning: prevention of uncontrolled
proteolysis" in "Protein purification methods - a practical approach", ed.
E.L.V. Harris, IRL Press, Oxford.
Jones E.W: (1991): "Tackling the protease problem in Saccharomyces
cerevisiae"; Meth. Enzym. 194, pp. 428-453.
Christian Velten
Institute for Molecular Biology, Medical School Hannover