IUBio

Urgent!endoproteinase Lys-C

Fuyuki Kametani kametani at PRIT.GO.JP
Tue May 9 20:21:09 EST 1995


---------------Original Message------------
Hi!
I(I4(Jm looking for a protocol for endoproteinase lys-C digest!
Any help will be appreciated!!

   Thanx
         Anne
----------End of Original Message----------


Previously, I carried out the digestion with the endoproteinase lys-C.
The protocol shows below.

A peptide (42 amino acid residues, 50 ug) 
(1) dissolved in 98 ul of 0.1 M ammonium bicarbonate solution, pH 8.2
(2) added 2 ul of endoproteinase lys-C solution (1 mg/ml)
           E/S = 1/25
(3) incubated at 37C for 3-7 hr

I hope that this protocol is of help to you.

-------------------------------------
Name: Fuyuki Kametani
E-mail: kametani at prit.go.jp 

Department of Moleclular Biology
Tokyo Institute of Psychiatry
Fax. Japan-3-3329-8035 
-------------------------------------
 




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