In article <95128.132452JPS144 at psuvm.psu.edu>, Joe Stains
<JPS144 at psuvm.psu.edu> wrote:
> Someone in my lab is running SDS-PAGE on a cell lysate. However, when
> they run the gel, the sample lanes spread out (longitudinally), pushing
> the adjacent lanes out as well. No one seems to have seen anything like
> this before. We've tried several things to reduce salt, getting rid of
> any lysis buffer, decreasing sample load volume, but nothing seems to
> work. We've even decreased the gel speed to hopefully correct the
> problem.
>
I believe you may have missed the obvious. Have you tried loading less
protein on the gel??
Darren
--
Darren Fast
Dept of Biochemistry
University of Wisconsin-Madison
ph. (608)-262-4705
fax (608)-262-3453
e-mail FAST at biochem.wisc.edu
