Dear Liz
It is best not to think of Scatchard plots as a way of *analysing* binding
data, but rather as a visual way of *displaying* them. This may seem a
fine point, but the visual impression can be very misleading compared to
the results of non-linear regression fitting of an appropriate equation on
to the data.
Two programs which will let you do this are:
Enzfitter (for PCs, from Elsevier)
Kaleidagraph (for Mac, from Synergy Software)
Both of them allow you to create/edit your own equations, which you can
then fit to the data - the statistics of the fit then give you a way of
deciding *objectively* which equation is the best model of the data.
Please email me privately if you want more detailed comments.
Simon Easterbrook-Smith
In article <3uod5q$5l8 at susscsc1.rdg.ac.uk>, sarfasha at reading.ac.uk wrote:
> I am attempting to do receptor-binding assays.
> I need some help on analysing my data using Scatchard plots.
> If anyone has any good PC/Mac programs that they can recommend or any
other help
> will be welcome.
>> Liz Fashola
> Post-grad,
> University of READING
>
--
Dr S B Easterbrook-Smith
Department of Biochemistry
University of Sydney
Sydney NSW 2006
AUSTRALIA
Phone (+) 612 351 3905
FAX (+) 612 351 4726
Email sbe @biochem.usyd.edu.au
