This procedure is not too reliable because od the contributions from
other side chains, notably cys-cys. But try Prasad et al (1986) Bichem.
25, 5214, using A280 and A215.
P.M.
On 21 Jul 1995, Michael Salvucci wrote:
>> Does anyone know of a method (or computer program) for accurately
> determining the concentration of a protein from the A280 and the
> sequence (i.e., the number of tyr & trp residues).
>> Thanks in advance,
>> Mike Salvucci, USDA-ARS
> Phoenix, AZ
>>msalvucc at asrr.arsusda.gov>>
