mesalvu at ix.netcom.com (Michael Salvucci) wrote:
>Does anyone know of a method (or computer program) for accurately
>determining the concentration of a protein from the A280 and the
>sequence (i.e., the number of tyr & trp residues).
>Thanks in advance,
>Mike Salvucci, USDA-ARS
>Phoenix, AZ
>msalvucc at asrr.arsusda.gov
The GCG package includes a program called pepsort that gives you a
calculated extinction cooefficent. It works well for me. The
reference is Gill, S.C. and von Hippel, P.H. Anal. Biochem. 182;
319-326 (1989)
