I'm in the process of setting up an assay system to see if a membrane protein
I've purified is a substrate for cAMP-dependent protein kinase (PKA).
Firstly, what concentrations of ATP should I be using? If I add [32P]ATP to
the reaction mixture the final concentration will be ~0.1uM. Would this be
enough to indicate preferred substrates for phosphorylation?
Any information would be gratefully received.
Thanks, Rob.
