IUBio

renaturation of enzymes

Hiranya Roychowdhury hroychow at NMSU.EDU
Thu Jul 20 09:27:56 EST 1995


Somebody asked about a protein that can be renatured with full activity. 
As a molecular biologist, the first one that comes to my mind (and I am
sure most live in dread of this one) is the Omnipresent and Omnipotent
'RNase'. Although I have not worked with its biophysical or biochemical
properties, it is known to renature with most of its activity from almost
every physical treatment, including the conditions used in autoclaves.
Only a combination of chemical and physical treatments can effectively rid
surfaces of this protein and/or its ability to renature. 


			>>>>>>>>>>>>>>>>>>>>>>>>>>>
			  Hiranya S. Roychowdhury
   			  Plant Genetic Engineering Lab.
			  Box 3GL, NM State Univ.
			  Las Cruces, NM 88003
			  Phone: (505) 646-5785
			  hroychow at nmsu.edu
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