Dear Netters,
I'm working on a protein kinase activity in barley aleurone vacuoles. It
appears to be membrane-associated. I'm trying to determine what effectors
might regulate it's action. So far, I've tried Ca, CaM, and different
hormone treatments. None of these appear to affect the kinase activity.
My question is: what other cellular regulators might modulate this
activity? I've not tried the cyclic nucleotides yet, but they're
candidates. How about DAG or IP3? This is kind of a general question, but
I'd appreciate any ideas you might have.
Thanks,
Simon.
