On 14 July on sci.chem.analytical, Wu Zhigang posted a discussion of the
above referenced title.
Has Wu Zhigang ever heard of cross flow field flow fractionation (FFF)?
This type of chromatography seems to share a lot of his discoveries and
"new concepts". The important feature of this type of FFF combined with
multiangle light scattering detection is that it permits for the case of
proteins, for example, an excellent separation in virtually ANY
aqueous-based mobile phase. Thus aggregation phenomena induced by the
requirement to use a mobile phase (e.g. PBS) that prevents protein
sticking to the column (e. g. SEC or GPC) can now be avoided. The
effective surface area of a cross flow FFF channel is miniscule compared
to a standard reverse phase or SEC column bed.
Incidentally, what type of chromatography is done throughout the various
universities and research centers of Singapore? Is there any place to
find out more details of such work? Is it true that the largest
biotechnology research center in Asia is located in Singapore?
Many thanks
Philip Wyatt
