Have you tried loading a pronase (0.5 mg/ml in buffer at pH7.5) solution and
letting the column sit in that for 24-48 h at 37 C then flush with buffer
(+/-SDS?)? This worked for me (-SDS) with reverse phase HPLC columns that
were clogged with clam proteins. Column resolution was restored. Note: when
doing this run the column backwards as suggested by another responder.
Goo luck (;
Neil W. Ross, Ph.D.
National Research Council Canada
Institute for Marine Biosciences
1411 Oxford St., Halifax, NS B3H 3Z1
(902)426-8402 FAX (902) 426-9413
Email: ROSSN at imb.lan.nrc.ca
