I'm trying to use an inducable system based on a Lac Repressor-Herpes
VP16 fusion protein. However, I really need to document expression of
this protein since functional assays are a pain and need to be reserved
for a few stable cell lines. A western would seem to be ideal but I am
having an unexpectedly difficult time in tracking down antibodies to Lac
I or VP16 (I've checked the usual sources without success). If you know
of any such antibodies, or better yet have an excess that you'd be
willing to share, please drop me a line.
Thanks in advance
Ed
Edward C. Goodwin Ph.D.
ecg at po.cwru.edu
Yale University Department of Genetics
