Hi Dear Netters:
Can anyone tell me how to recover a clogged HIC cartridge, which is an
Econo-Pac Methyl HIC cartridge made by Bio-Rad? I'm using it to purify a plant
apoplastic protein. I broght the infiltration solution to 2.4 M ammonium
sulfate and applied it to the cartridge. The proteins were eluted with a linear
gradient of ammonium sulfate from 2.4 M to zero. The specific enzyme came out
at the end of gradient. In the beginning, the flow rate was about 1 ml/min.
However, after several uses, the flow rate was reduced to 1 ml/hr. I did clean
the cartridge as suggested by the manufacturer, using 100mM NaOH, 20% glycerol,
and deionized water to wash. And I even washed it with 0.5M NaOH and 70%
ethanol. They all didn't work. Please tell me what else I can do. Thanks a lot
in advance.
Ming
hsieh.21 at ous.edu
