>> More troublesome is that the fusion of the
>> same protein to the maltose-binding protein
>> (MBP) is sticking to the walls of the plastic
>> eppendorf tubes after HPLC purification.
>>More pure a hydrophobic protein the more will be its propencity to bind
>to plastic (hydrophobic) surfaces. Try siliconizing.
>Doesn't siliconizing meking the surface even more hydrophobic?
I would try a glass tube instead.
Wolfgang
