Triton-X 100 effect on protein concentration determination

[Anne Spang]

Hi!
Triton X-100 messes up your Bradford assay! In the presence of detergent
in our sample, we usually precipitate the sample with TCA in a dotblot
apparatus on nitrocellulose, then stain with amido black. After elution
teh samples can be read in an ELISA reader or a normal photometer.

Hope this helps
      Anne