Jim Litts (jlitts at onyx-pharm.com) wrote:
[asking for E. coli strains with protease deletions]
BL21 and derivatives are E. coli B strains which are protease-deficient
compared to WT E. coli K-12 (e.g. they do not contain outer membrane
proteases)
There are several lon-negative E. coli K-12 strains. lon is an ATP-
dependent protease. K-12 which are deficient in the heat-shock
response (sorry, can't recall genotype) are also lon-deficient.
Some of these strains are available from NEB.
--Cornelius.
--
/* Cornelius Krasel, Abt. Lohse, Genzentrum, D-82152 Martinsried, Germany */
/* email: krasel at alf.biochem.mpg.de fax: +49 89 8578 3795 */
/* "Science is the game you play with God to find out what His rules are." */
