Hi,
I recently used the GST fusion system to purify my protein (a
cellulase). Everything went fine up to the stage of gel filteration and
I had a nice single band . Now the misery part is that my protein which
is about 57kD degrades to two low molecular weight products which are
still active on zymograms. Does this mean my prep is contaminated with
proteases or I am experiencing some form of self digestion judging from
the fact that my protein was pure initially? And how can I prevent
this? I need help before my patience runs out.
Abiye
