In article <3ga5j2$omq at emory.mathcs.emory.edu> rmiller at bimcore.emory.edu (Richard Miller) writes:
>From: rmiller at bimcore.emory.edu (Richard Miller)
>Subject: Re: Antibodies to phosphorylated amino acids
>Date: 27 Jan 1995 06:58:42 GMT
I can echo the lack of specifity of Sigma's phosphoserine antibody. We use
brain tissue and we get a large number of bands that represent non-specific
staining. Another problem is background blocking. Using non-fat fry milk is
impossible since it contains phosphoserine proteins. Using only tween-20
gives horrible backgrounds. We are trying BSA/tween-20 which helps some, but
we are nowhere near publishable quality pictures.
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Gary Krause (gkrause at cms.cc.wayne.edu)
Department of Emergency Medicine
Wayne State University, Detroit, MI USA
