lenny at zeus.datasrv.co.il (Lenny Garfinkel) wrote:
>This group appears to be dead, but I guess I can't lose by posting. I
>have a fermentation supernatant (does not matter what organism) which I
>suspect contains proteases. When I add a pure protein to the sup and
>incubate for anywhere from 0.5 hr to overnight, I see an apparent
>INCREASE in molecular weight on SDS-PAGE in the presence of
>mercaptoethanol (yup, it runs slower). What sort of protein
>modifications could cause this? Could clipping of a few residues result
>in less SDS binding?
>Yes, especially if you chop off a bit of positively charged sequence
>
__
Jonathan B. Marder '
Department of Agricultural Botany | Internet: MARDER at agri.huji.ac.il
The Hebrew University of Jerusalem | /\/
Faculty of Agriculture |/ \ Phone: (08 or +9728) 481918
P.O.Box 12, Rehovot 76100, ISRAEL / Fax: (08 or +9728) 467763
