In article <1995Jan19.145157.7473 at nki.nl>, gbrink at nki.nl wrote:
> Dear All,
>> Who can tell us how to link one single T to a blunt-end DNA fragment, to
create
> a sticky T-overhang ?
>> Guido Brink &
> Arno Floore
> Netherlands Cancer Institute, Amsterdam
Incubate the DNA with Taq polymerase (1 U/ ug plasmid/ 20 ul volume) and 2
mM dTTP in 1X Taq buffer for 2h @ 70°C. Then phenol extract and EtOH ppt.
See also Marchuk et al, Nucleic Acids Res 19(5), p. 1154.
Shane Albright
MCB Dept., UC Berkeley
Berkeley, CA
