This group appears to be dead, but I guess I can't lose by posting. I
have a fermentation supernatant (does not matter what organism) which I
suspect contains proteases. When I add a pure protein to the sup and
incubate for anywhere from 0.5 hr to overnight, I see an apparent
INCREASE in molecular weight on SDS-PAGE in the presence of
mercaptoethanol (yup, it runs slower). What sort of protein
modifications could cause this? Could clipping of a few residues result
in less SDS binding?
--
_________________________________________________________________
Dr. Leonard Garfinkel | Internet: lenny at zeus.datasrv.co.il
Bio-Technology General | Office Phone: 972-8-381256
Kiryat Weizmann | Home Phone: 972-8-451505
Rehovot, Israel | FAX: 972-8-409041
-----------------------------------------------------------------
