Sarah Swanson (swanson at nature.berkeley.edu) wrote:
> I'm looking at the possibility of using antibodies to phosphorylated amino
> acids as an alternative to radiolabelling in studies of protein
> phosphorylation. Has anyone got any tips/comments about this, especially
> regarding the specificity of the antibodies.
Phosphotyrosine antibodies apparently work well. There is a phosphoserine
/ -threonine antibody on the market (Sigma) but AFAIK its specificity is
lousy.
--Cornelius.
--
/* Cornelius Krasel, Abt. Lohse, Genzentrum, D-82152 Martinsried, Germany */
/* email: krasel at alf.biochem.mpg.de fax: +49 89 8578 3795 */
/* "Science is the game you play with God to find out what His rules are." */
