I have one particular protein that, when expressed as a GST-fusion protein,
has a great deal more difficulty with degradation than others. I was
wondering what cell lines people are working with that might be useful for
stabilizing such proteins, hopefully while still maintaining a reasonable
level of expression. Perhaps with endogenous protease deletions. It seems
I remember having some such lines several years back in another lab, but I
can't find my records of them. If you respond by Email, I will summarize
and post the information. Thanks.
Jim
