In article <r.k.mcewen.30.0012D5FE at bham.ac.uk>, r.k.mcewen at bham.ac.uk (ANON) writes:
> Recently I`ve been observing an artefactual band on my SDS-PAGE gels
> regardless of whether there is any samples on it. This band typically runs
> between 50 and 60 kDa right across the gel, through all the sample lanes and
> varys in intensity from one to another.
> To date, I have tried fresh reagents; a colleagues reagents and gel equipment
> all to no avail.
> Needless to say, he does not see this band. I have tried to mimic his method
> of preparing the gel but I am at a loss to explain it.
>> Any suggestion ?
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Maybe you could elaborate a little: how are you visualizing your bands?
Does your unwanted band show up only after staining, or is it radioactive,
or is it visible without staining in the gel itself? Is in confined to
gel lanes, or does it also show in the spaces between lanes? Do you always run
the sample loading buffer dye-front off the gel?
Cheers,
Kevin.
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Kevin W. Ryan
Department of Virology & Molecular Biology
St. Jude Children's Research Hospital
Memphis, Tennessee 38101-0318, U.S.A.
phone: (901) 522-0411
fax: (901) 523-2622
Internet: ryan at mbcf.stjude.org
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