In article <r.k.mcewen.30.0012D5FE at bham.ac.uk>, r.k.mcewen at bham.ac.uk
(ANON) wrote:
> Recently I`ve been observing an artefactual band on my SDS-PAGE gels
> regardless of whether there is any samples on it. This band typically runs
> between 50 and 60 kDa right across the gel, through all the sample lanes and
> varys in intensity from one to another.
> To date, I have tried fresh reagents; a colleagues reagents and gel
equipment
> all to no avail.
> Needless to say, he does not see this band. I have tried to mimic his method
> of preparing the gel but I am at a loss to explain it.
>> Any suggestion ?
Yes. Try not to touch anything, including your stock solutions, without
wearing gloves. You are probably seeing bands that correspond to the
keratins from your fingers.
--
mwd
