Recently I`ve been observing an artefactual band on my SDS-PAGE gels
regardless of whether there is any samples on it. This band typically runs
between 50 and 60 kDa right across the gel, through all the sample lanes and
varys in intensity from one to another.
To date, I have tried fresh reagents; a colleagues reagents and gel equipment
all to no avail.
Needless to say, he does not see this band. I have tried to mimic his method
of preparing the gel but I am at a loss to explain it.
Any suggestion ?
