lmarekov at DXI.NIH.GOV wrote:
: Does anyone know the chemistry for derivatizing PVDF membranes
: to Arylamino-PVDF for sequencing purposes?
: Millipore used to sell them as Sequelon-AA, but they have been
: out of stock for more than two months.
: Lyuben Marekov
: NIH/NIAMS, Bethesda MD 20892
:lmarekov at dxi.nih.gov
hello Lyuben,
you might check the paper of Rodrigues, Combrink and Brandt
in Anal. Biochem. 216:365 (1994). At least as far as I recall
they modify a PVDF membrane for covalent coupling of peptides.
Best wishes
Mil
--
Emile Schiltz <eschiltz at sun1.ruf.uni-freiburg.de>
or <schiltz at bio2.chemie.uni-freiburg.de>
Institute of Organic Chemistry and Biochemistry
Albertstrasse 21, D-79104 Freiburg, Germany
phone: +49 761 203 6090
