In article <3fdfm9$hhn at mserv1.dl.ac.uk>
vachon at bio.grenet.fr (Gilles VACHON) writes:
> Dear netters,
>> I am having trouble in getting regular amounts of pET (19b or 11a, with ot
> without insert)) in DH5alpha.
> I have only 30-50ug of plasmid from a 200ml LB culture (Cesium Chloride prep).
>> The DH5alpha strain is ok since I get 500-700ug of pUC in the same conditions.
>> Does anyone on the net have an answer to that problem ?
> I am planning to do cloning with these pETs and I bet I won't be able to
> check the clonings with that low yield.
>> Thanks
> Dr. Gilles Vachon
> Laboratoire de Biologie Moleculaire Vegetale
Same with me. pET is a low copy number plasmid. Did anybody try
chloramphenicol amplification?
Serge
