Gilles VACHON (vachon at bio.grenet.fr) wrote:
> I am having trouble in getting regular amounts of pET (19b or 11a, with
> or without insert) in DH5alpha.
> I have only 30-50ug of plasmid from a 200ml LB culture (Cesium Chloride
> prep).
I'm afraid that's normal. I have the same problems with pET3* in JM109.
(pET isn't exactly a high-copy plasmid ...)
--Cornelius.
--
/* Cornelius Krasel, Abt. Lohse, Genzentrum, D-82152 Martinsried, Germany */
/* email: krasel at alf.biochem.mpg.de fax: +49 89 8578 3795 */
/* "Science is the game you play with God to find out what His rules are." */
