In article <3eit1e$o29 at nntp1.u.washington.edu>, Bassuk at U.Washington.Edu (Jim Bassuk) says:
>>In standard chromatography of proteins, we monitor the absorbance of the column eluate with a
>Pharmacia UV-1 flow cell coupled to a chart recorder. This analog signal from the UV-1 is 10 mV.
>>To design a system to convert the signal to a digital format, I'll need a A/D converter, some sort
>of board for a yet-to-be purchased 486 PC, and some sort of software to acquire, process, and store
>the data.
>>We'll likely couple two UV-1 units in tandem (214 and 280 nm).....this is 2 channels.....and then
>a simple closure switch to indicate the advancement of the fraction collector....this is another channel...
>a total of 3 channels.....
>>No need to buy a big, expensive HPLC software program, because I won't be controlling any pumps,
>etc...
>>Comments on boards, A/D units, and software sources and experiences are respectfully requested.
>>Thanks in advance,
>>Jim Bassuk
>BioStructure
>Univ Washington
Dear Jim,
By the time you purchase two UV-1 detectors you will probably
have spent more than it would cost to buy a very good dual channel
continuously variable detector. There are many dual wavelength
detectors on the market such as Gilson, Waters, Isco. You might even
want to consider a diode array detector which won't be that much more
than the dual wavelength. Sorry I cant help with the data aquisition
software.
Best Regards.
John Marcus
