maga at vetbio.unizh.ch (Giovanni Maga) wrote:
stuff deleted
> Can you use Hill plot as a test
> for real cooperativity? Or it will give you a h coeff. different from 1
> even in those cases in which the non-linearity has nothing to do with
> cooperativity (for example if your enzyme has alternative pathways for
> binding two molecules of substrate with no irreversible steps in between)?
> And if so, how can I convincingly prove (or disprove) cooperativity?
You are absolutely right that a sigmoidal plot will give a Hill slope
greater than 1 whether you have positive cooperativity or some other
mechanism or artifact causing the sigmoid nature. A bit more information
about the experimental design might help.
Rick Neubig
http://www.umich.edu/~rneubig
