In article <3euc3i$3ib at sunserver.lrz-muenchen.de>, u7q11aa at sunmail.lrz-muenchen.de (Peter Alliger) writes:
>I am looking for good method to precipitate a highly purified protein
>before
>analysing it on a SDS-PAGE.
>The fraction has about 1M KCl and should be concentrated from 500 ul to
>about 20.
>>Thanks for suggestions
>>PIT
>>===============================================================
>Peter Alliger, Ph.D.
>Institute for Immunology home address:
>University of Munich
>Goethestr. 31 Maximilian-Wetzger-Str.7
>D-80336 Munich, Germany D-80636 Munich, Germany
>>Phone:(49-89)5996-673 (49-89)183426
>Fax: (49-89)5160-2236
>----------------------------------------------------------------
>Why not prcipitate with 8X volume of cold acetone (at -20C) for a 1 h and
redissolve the ppt in sample buffer required for SDS-PAGE. Good Luck. Abul
EKRAMODDOULLAH, ABUL KALAM M. Title: Research Scientist
Forestry Canada Phone: (604) 363-0600
Victoria, B.C. Internet: AEKRAMODDOUL at A1.PFC.Forestry.CA
