J.Marcus at tpp.uq.oz.au (John Marcus) writes:
>>I am looking for good method to precipitate a highly purified protein
>>before
>>analysing it on a SDS-PAGE.
>>The fraction has about 1M KCl and should be concentrated from 500 ul to
>>about 20.
>>>>Thanks for suggestions
>>>>PIT
>>>Have you thought about using a Centricon or Microcon centrifugal
>filtration device. Essentially, you put your protein sample into
>the microcon (~500ul capacity) and then spin in a microcentrifuge
>for 1 min to 30 min (depending on the molecular weight cutoff of
>the filter that you are using at the time). I think Amicon may
>have molecular weight cuttoffs as low as 1000Da. When you are done
>spinning you should be left with about 10ul volume if you use a
>microcon; although, this holdup volume is not very predictable.
============
An excellent suggestion, especially since the KCl will have to be removed
or reduced to prevent precipitation of the SDS.
--
Eric Larson | University of Illinois at Urbana-Champaign
USDA/Agronomy | 190 PABL; 1201 W. Gregory; Urbana, IL 61801
elarson at ux1.cso.uiuc.edu | Voice 217.244.3079 Fax 217.244.4419
Fidonet: 1:233/4.1 | My opinions are my own, but correct :-)
