Does anyone have experience of using crossed immunoelectrophoresis for the
study of glycoforms of protein ?
I am currently working on the microheterogenity of acid glycoprotein using
concanvalin A in the first dimension but having problems in getting the method
to work. I think my problem is the first dimension i.e. with the con A.
Does anyone have a method for using con A in CIE with regard to buffers etc.
I would appreciate any help on this matter.
TIA
--
Mr Craig Webster, Department of Chemical Pathology and Immunology
The Old Medical School, Thoresby Place, Leeds, LS2 9JT
Tel:- 0532 923285 email:- craigw at pathology.leeds.ac.uk
Fax:- 0532 335672 mphcw at gps.leeds.ac.uk
