In article <3eg2t9$8hl at neuro.usc.edu>, william at neuro.usc.edu (Fiberman) writes:
>> Hello, Netters,
>> Can a western blot filter be "reprobed" like a Southern blot filter?
Yes, it can. Use the following stripping buffer: 100 mM 2-ME, 2% SDS, 62.5 mM
Tris.HCl pH 6.7. Submerge membrane in buffer at 50 deg C for 30 minutes. Wash
twice for 10 minutes in your usual wash buffer. Reblock. Reprobe.
I have used this several times and it works.
Ian Davis davis at licre.ludwig.edu.au
Ludwig Institute for Cancer Research
Melbourne Tumour Biology Branch
Melbourne, Australia.