In article <3eg2t9$8hl at neuro.usc.edu>, william at neuro.usc.edu (Fiberman) wrote:
> Hello, Netters,
>> Can a western blot filter be "reprobed" like a Southern blot filter? If
> so, what is the best way to strip the original antibody? I am using a
> secondary antibody conjugated to alkaline phosphatase, and visualizing
> with addition of color substrates NBT and BCIP. I would appreciate your
> response.
>> -fm
I think this method causes a precipitate to form, and you can't strip
and reprobe when using this method.
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