Being a newcomer to protein purification, I read about an enzymatic
method of cell lysis of E.coli that uses sucrose and spermidine
(Meth. Enzym. 182:149) but the scale is much too high for the
amount of cells I have (pellet from ~2l).
Is there a similar method available that is appropriately scaled
down? Or should I use the above method at 1/200 volumes? Thanks
in advance.
- Dale Yuzuki M.A. M.Ed. - John Wayne Cancer Inst. - dale at jwci.org - __@
- 2200 Santa Monica Blvd - Santa Monica CA 90404 - (310) 449-5267 _ -\<'_
- I do not pray for success. I ask for faithfulness. Mother Theresa(_)-/-(_)
