I have recently purified several transcription factors of the basic-domain/
leucine zipper class from a bacterial expression system. I was wondering
leucine zipper class from a bacterial expression system. Several of these
bZIP factors can form weak heterodimers and I was wondering if there is
an appropriate protocol for measuring the relative affinity of each
subunits for each other (something like a Km?).
Any suggestions are welcome. Thanks.
Andre Nantel
Biology
University of North Carolina
