Hello,
I am interested in using the two-hybrid system to search for proteins which
interact with the protein I study. The target protein in this case is secreted.
I have two questions concerning this situation. (1) Is there available a Gal4
DNA binding domain vector which adds a nuclear localization signal to the fusion
protein? (2) Does the library need to be constructed in an activation domain
plasmid which also has nuclear targetting adaptations or does the activation
domain of Gal4 provide the localization already? Thanks, Kevin.
