In article <2r0b7e$hga at sun4.bham.ac.uk>, J.E.Fox at bham.ac.uk (John E. Fox) says:
>>In article <2qtk1j$nnn at usenet.INS.CWRU.Edu>, evw2 at po.CWRU.Edu (Eric V. Wong) says:
>>>>Dear Eric,
>>In reply to your Email:- this is what we can do in the field of peptide synthesis
>, plus everything else. Just give me a call if you are interested.
>>>Alta Bioscience, the essentials:-
>>Synthetic methods
>Oligonucleotide synthesis
>* Rapid synthesis of purified oligos ready for use in PCR or
> sequencing.
>* Synthesis on 0.2 and 1.0 micromole scales.
>* Larger scales of synthesis.
>* Mixed base oligos.
>* Doped oligos.
>* Fluorescein or Biotin tags at any position.
>* Phosphorothioate oligos.
>* Oligos coupled to solid supports.
>* Optional reverse phase cartridge purification.
>>Peptide synthesis
>* Laboratory scale synthesis, 50 to 500 micromole amounts of
> peptides.
>* Multiple peptide synthesis: 30 micromoles, 12 peptides
> simultaneously.
>* Multiple peptide synthesis: 5 micromoles, 48 peptides
> simultaneously.
>* Multiple antigenic peptides, MAPs, (octomeric peptides).
>* Peptides supplied crude or purified.
>* Quality control includes amino acid analysis, HPLC, mass
> spectrometry.
>>>Sequencing techniques
>DNA sequencing
>* Approximately 400 bases per reaction, depending on template
> quality.
>* Sequencing of plasmids or PCR products.
>* Can use ordinary primers.
>* Only needs 1 micro gramme of template, (for a plasmid).
>* Data presented as hard copy or in electronic form.
>>Protein micro sequencing
>* High sensitivity, capable of sequencing at the pico mole level.
>* Can sequence proteins or peptides.
>* Can sequence either proteins in solution or on PVDF blots.
>* Radio sequencing available.
>* Rapid return of data.
>>Analytical methods
>Amino acid analysis
>* Amino acid analysis on virtually any type of material.
>* Separation of either protein hydrolysates or biological fluids.
>* All sample preparation included.
>* Choice of detection systems.
>>>Laser desorption mass analysis
>* Suitable for a wide range of molecular weights of up to 200kDa.
>* Capable of processing large numbers of samples.
>* Picomole sensitivity.
>* Mass measurement accurate to *0.04%.
>>>High Performance Liquid Chromatography
>* Range of columns available.
>* Choice of detectors, UV, RI, fluorescence, electrochemical.
>* Analytical or semi preparative scale of separations.
>>>>>>For further details on these methods please contact the Director:-
>>Dr. John E. Fox
>School of Biochemistry
>The University of Birmingham
>Edgbaston
>Birmingham
>U.K.
>>Tel 021 414 5450
>Fax 021 414 3376
>>
