In article <0097DE31.958FC4A0.28681 at lsm.nrl.navy.mil> miller at LSM.NRL.NAVY.MIL writes:
>Hello everyone,
>>I am currently trying to overexpress a eukaryotic protein using a T7 RNA
>polymerase promotor system, but am only getting about 1 mg/liter culture of
>expressed protein. I am looking for ways to optimize this expresAny
>>can determine which codons are most frequently used in E. coli. Any other
>suggestions would be greatly appreciated.
For a table of codons look in the Novagen Catalogue, 1994, p68 (Ref Gribskov
et al NAR 1984, 12, 539-49). For an example of increasing expression of a
gene see Holler et al, Gene 1993, 136, 323-8.
Joe Mack, BSc
mack at ncifcrf.gov
>>Charles Miller, PhD
>
